Standard sybr green i dye reaction note. Whether you purchased commercially available primers or created your own using our protocol design your own qpcr primers it is important to test the primers using your samples your sybr green master mix and your qpcr machine to be certain that you are amplifying a single amplicon and that it is the proper sequence for the mrna you are trying to measure. Iq and ssofast pcr supermixes have been supporting researchers performing basic real time pcr analysis for years.
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