Sybr Green Qpcr Mix

Many pcr machines require a passive reference dye.

Sybr green qpcr mix. Sybr green master mixes are available with other dyes including rox. Direct detection of pcr product is monitored by measuring the increase in fluorescence caused by the binding of sybr green dye to double stranded ds dna. In routine pcr the critical result is the final quantity of amplicon generated after the process. Dye based quantitative pcr qpcr uses real time fluorescence of a double stranded dna dsdna binding dye most commonly sybr green i to measure dna amplification during each cycle of a pcr.

Applied biosystems power sybr green pcr master mix delivers highly sensitive nucleic acid quantitation detecting as few as 2 copies of a target gene over a broad range of template concentrations. The jumpstart taq antibody inactivates the dna polymerase at room temperature. This kit contains the high performance mircury sybr green master mix for quantitative real time pcr amplification. The master mixes contain buffer dntps thermostable hot start dna polymerase and of course sybr green dye everything needed for real time pcr except the sample and pcr primer pair.

Lna enhanced primer sets require specific pcr reaction conditions for optimal performance. Simply add 25 μl of the 2 mix to dna template primers and water. This ready to use mixture of sybr green i jumpstart taq dna polymerase 99 pure deoxynucleotides and reaction buffer is provided in a 2 concentrate for ease of use. Sybr green master mixes are 2x concentrated and contain dntps mgcl 2 and dna polymerase.

Itaq universal sybr green supermix is a 2x concentrated ready to use reaction master mix optimized for dye based quantitative pcr qpcr on any real time pcr instrument. The newly optimized formulation contains highly purified applied biosystems amplitaq gold dna polymerase ld to offer greater sensitivity. Applied biosystems sybr green master mixes are designed for quantitative real time pcr using a set of two pcr primers that flank the target region. The sybr green pcr master mix is a convenient premix of the components except primers template and water necessary to perform real time pcr using sybr green i dye.

An internal reference dye such as rox corrects well to well optical variations and is used for fluorescent signal normalization. The mix prepared at 2x reaction concentration can be directly used for robust and low template quantitative pcr with.